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I have used this for TC amplification with good results, http://www.analog.com/en/prod/0,2877,AD595,00.html has built in CJC, as long as you keep the TC termination as close to the IC pins you're golden.

doh, I also forgot, the other constraint for the single line is there is a single input to the card, and that is pretty much frozen.

thanks for the response, my real problem is I have no time to prototype this, but need a well designed text description. I really hate engineering this way but sometimes thats how it goes. I certainly know things never work as well at the bench as on paper. Im getting from your response that...

I am trying to spec a project, general theory of operation: have 4 fluids: air(sterile), and 3 aqueous solutions use the air to flush a (semi) ufluidics card between reagents the first two reagents are ~mL washes through the entire card (Q=1200uL/min <or less is ok> ), spec grav~ 1.1 , 0.9 -...