At the very end of the pipe, velocity will approach sonic condition. Choked flow will prevail, and the polytropic flow equation will geve a pretty good idea of the maximum oor "ideal" flow.
The actual condition will be somewhat less because you do not have a "clean" orifice, that is the flow will have the chance to develop in the immediately upstream portion of the pipe and thus there will be a velocity profile immediately upstream of the outlet, the portions nearest the pipe wall travelling slower than sonic...The density in this portion is also varying, so that analysis is more involved, but it can be solved as a function of length based on the assumption of constant entropy using ideal gas equation.
If the length of the 1/4" pipe is more than a few diameters, the result from the choked orifice equation will be significantly higher than actual.
This is probably the only portion of the pipe assembly you will need to analyze...Unless the upstream components are of similar size, its doubtful they will have any significant effect on the choke condition; they will represent a minor percentage of the total available pressure difference.