reconvolution
Bioengineer
- Jun 6, 2010
- 11
Colleagues,
I'm setting up a fluorescence experiment with quinine as fluorescent dye. Quinine is a common fluorescence standard, that's why I chose it. The caveat with quinine, though, is that its excitation band is in near UV; peak is between 320nm and 370nm. Due to size constraints, I'd like to use LED for excitation. At the moment I don't know the minimum amount of illumination that will be needed.
First, I've looked for LEDs with wavelength peak in the desired range. The LEDs I was able to find are low power – under 1mW. In terms of flux, it's 10s of mlm vs. 1000s of mlm delivered by high power blue LEDs (e.g. Osram Golden Dragon).
Currently, I'm looking for high power visible light LEDs with broad tails extending into UV. Haven't found a suitable one yet.
Any suggestion or insight is really appreciated!
- Nick
I'm setting up a fluorescence experiment with quinine as fluorescent dye. Quinine is a common fluorescence standard, that's why I chose it. The caveat with quinine, though, is that its excitation band is in near UV; peak is between 320nm and 370nm. Due to size constraints, I'd like to use LED for excitation. At the moment I don't know the minimum amount of illumination that will be needed.
First, I've looked for LEDs with wavelength peak in the desired range. The LEDs I was able to find are low power – under 1mW. In terms of flux, it's 10s of mlm vs. 1000s of mlm delivered by high power blue LEDs (e.g. Osram Golden Dragon).
Currently, I'm looking for high power visible light LEDs with broad tails extending into UV. Haven't found a suitable one yet.
Any suggestion or insight is really appreciated!
- Nick