culture media to grow SRB bacteria

[karimprocess]

Good afternoon,

i'm a junior corrosion engineer in a local oilcomapany.

in our fields we are facing bacterial activity and all the problems coming with it , especially SRB .
since we have some issues ordering SRB test kits (SRB vials) ; i wantto propose to my management to try and produce our SRB vials ourselves ; i mean producing our media in our lab according to NACE Standard TM0194-2004.

it is quite clear what are the diffrent ingredients to make the media mixture , but not if it should be bottled in a nitrogen environment, how to adjust the salinity of the mixture , which salt... , so i wanted to ask if some of you have already done this and if he can help me by telling how to do this procedure step by step.

Best regards.
abdul

 

[IRstuff]

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[james hops 101]

Hello Abdul,

Producing your own SRB test kits can be a cost-effective solution, but it's important to ensure that the media is properly prepared and bottled to maintain its quality and effectiveness. Here are some general steps to follow:

Obtain the necessary ingredients and equipment: You will need to obtain the ingredients required for the media mixture, including sodium thiosulfate, ferrous sulfate, magnesium sulfate, calcium chloride, and a carbon source (e.g. lactate). You will also need to obtain the necessary equipment, including glassware, a pH meter, and an autoclave.

Prepare the media mixture: Follow the NACE Standard TM0194-2004 to prepare the media mixture. Adjust the salinity of the mixture using the appropriate salt(s) to match the salinity of the environment where you will be testing for SRB. Use a pH meter to ensure that the pH of the mixture is within the appropriate range.

Sterilize the media: Autoclave the media at the appropriate temperature and pressure to sterilize it.

Bottle the media: Use sterile techniques to bottle the media in vials or other containers. Consider using a nitrogen environment to minimize contamination.

Quality control: Conduct quality control checks to ensure that the media is effective for testing SRB. This may involve conducting positive and negative controls to validate the media's effectiveness.

It's important to note that the specific procedures for producing and bottling the media may vary depending on the particular SRB test kit you are using. Be sure to carefully review the manufacturer's instructions and consult with experienced colleagues or a subject matter expert to ensure that you are following appropriate procedures.

I hope this helps. Let me know if you have any further questions.

 

[EdStainless]

James is spot on, this is bio lab work where clean and sterile is very important.
I imagine that you can buy the media blend from a biological supply house.
There are dozens of different growth media tailored for different organisms.
Compounding your own gets much more difficult as even small variation will impact the results.
And the various pure raw ingredients are not simple or cheap either.
Then it a matter of mixing with high purity water (very high purity) and placing into clean vials and autoclaving.
You will have to have a QA procedure in place for testing samples from each lot to assure that they work the way that is intended.

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