There are standards published by NEBB (National Environmental Balancing Bureau) as on how ducts of various sizes should be divided for a pitot tube traverse. Round ducts are treated differently than square. You may be able to get a professional air balancer to do this for you. Contact any large HVAC or balancing firm.
In a traverse, the duct is divided into sections small enough to be sure that enough data points are taken to ensure a representative sampling. For round ducts, samples are usually taken 90 degrees apart, and distances calculated so each position represents an annular region of equal area.