You can use combinations of ply thickness if you want, at the price of inventory and complication (if it's prepreg you get also risks like different combinations of out-life, though that sort of thing can happen anyway, and if it's infused in-plane different plies can have different permeabilities).
There are no particular mechanical property penalties in mixing and matching that I'm aware of. Others may know better.
Re k vs. areal weight, this depends on the fabric supplier (and possibly on the fiber supplier). Tows can be spread out before being woven together within limits. The tows in a typical typical fabric aren't round, they're spread out something like 5 or 10:1 in a flattish somewhat oval shape. I'm not sure how this sort of thing affects mechanical properties.
If you want a cured ply thickness of 0.25 mm (10 thou) and the material is from a commercial suppier who deals with 320k tow, then it needs to be spread out something like 20:1.
Not sure how good fabric suppliers are at bunching tows up.
One other practical point about ply thickness, thinner plies drape more easily.