As Yorkman said, your low pressure would be your "fill" pressure, which for a typical, non-high rise, building would be around 12 psig. Your high pressure can really be anything, as long as it's not above your relief valve setting. The higher you go, the smaller the required tank size will be. As you know from the equation, it's a function of water volume, temps, etc. Make sure you use psia and not psig in the calcualtion.
Now, you mention that you have a back pressure regulating valve set at 50 psig. I don't know why a back pressure regulator would be on a hydronic system????? But if that is your lowest relief valve setting, then you must make it your high pressure in the expansion tank calculation. Otherwise, the tank will be too small and the system pressure will reach the backpressure setting.
Regarding your pump, you are thinking of an open system. You don't have to worry about friction loss, etc. Hopefully you have the expansion tank right on the suction side of the pump? That's the best way to go in my opinion. But the bottom line is you're trying to calculate the maximum pressure. What you need to do is determine what max pressure is allowable. In reality you may never see it. So I would use 50 psig (64.7 psia) in the equation.
Yorkman, do you agree?