dgidwani,
The term "one truck per lane" refers to the to the longitudinal direction or "end to end" (NOT side by side).
AASHTO is specific in section 3.11.4 when stating that, FOR CONTINUOUS SPANS, one truck load per lane shall be considered ON THE STRUCTURE (therefore, KOODI's first post is correct because he qualifies it with "continuous" spans. From my point of view, the omitting of simple spans in the statement implies that as many truck loads (aka axle loads) shall be placed ALONG a 12 foot lane (in the longitudinal direction) to produce the maximum stress for whatever component you're designing.
AASHTO 3.6 addresses Traffic Lanes and basically specifies that you don't put more than one truck or lane load within a 12' lane WITH REGARD TO THE TRANSVERSE DIRECTION (i.e. if you have a 35'-11" roadway width, you can only place two 12' lanes on the roadway and each truck or lane load must be within their respective 12' wide lane.
KOODI's second post seems a little misleading (sorry KOODI). AASHTO states that LANE LOADS EQUAL TRUCK TRAINS (multiple trucks though not necessarily multiple HS20 loads)
however, the reduction for multiple lanes loaded is to account for the improbability of truck/lane loads side by side AT THE SAME LOCATION (longitudinally) THAT PRODUCES MAXIMUM STRESS IN THE COMPONENT - not just "on the bridge".
In conclusion, I would reiterate that you should place a truck load in each adjacent span, as close to the pier as possible, to determine the max reaction on the pier due to truck loads.
Also, with regard to ANDYZ's post, you would only use an impact coefficient based upon average span lengths of adjacent spans if the structure was continuous, otherwise, use individual impact coeff. based upon the span length of the individual simple span length.